TB-500

Description

TB-500 is a synthetic peptide corresponding to the active region of Thymosin Beta-4, a naturally occurring 43-amino-acid protein that plays a fundamental role in cellular processes related to tissue protection and repair. Thymosin Beta-4 is one of the most abundant intracellular proteins and is found in nearly all cell types, with particularly high concentrations in blood platelets and wound fluid. TB-500 represents the bioactive fragment of this protein, specifically the region responsible for actin binding and cell motility regulation.

The primary mechanism through which TB-500 exerts its effects in research models involves its interaction with globular actin (G-actin). By sequestering G-actin monomers, TB-500 modulates actin polymerization, which directly influences cell migration, proliferation, and differentiation. This actin-regulatory function is central to wound healing processes, as migrating cells require dynamic cytoskeletal reorganization to reach injury sites. In vitro studies have demonstrated that TB-500 promotes keratinocyte and endothelial cell migration in a dose-dependent manner, providing mechanistic support for its observed effects in whole-organism models.

Preclinical wound healing research has produced substantial data on TB-500's activity. In dermal wound models, researchers have documented accelerated wound closure, increased collagen deposition, and enhanced angiogenesis at the wound site. Cardiac tissue studies have been particularly noteworthy: research in murine models of myocardial infarction has shown that TB-500 treatment is associated with improved cardiac function, reduced scar formation, and activation of cardiac progenitor cells. These findings have generated significant interest in TB-500 as a research tool for studying cardiac regeneration.

Anti-inflammatory properties of TB-500 have been documented across multiple experimental paradigms. The peptide has been shown to downregulate inflammatory cytokines, including interleukin-1 beta and tumor necrosis factor alpha, in tissue culture models. In joint inflammation studies, TB-500 treatment was associated with reduced inflammatory cell infiltration and preservation of cartilage structure. Researchers studying musculoskeletal injuries have used TB-500 to investigate tendon and ligament repair, reporting improved tensile strength and fiber alignment in treated specimens.

TB-500 is supplied as a white lyophilized powder with purity exceeding 98% as confirmed by HPLC. Each vial contains 5mg of peptide and should be reconstituted with bacteriostatic water before use. The high molecular weight of the complete peptide sequence ensures biological activity consistent with published research protocols.

Composition

• Peptide: TB-500 (Thymosin Beta-4 Fragment)
• Molecular Formula: C212H350N56O78S
• Molecular Weight: 4963.44 g/mol
• Purity: >98% (HPLC)
• Appearance: White lyophilized powder
• Quantity: 5mg per vial

Storage

• Store lyophilized peptide at -20C for long-term storage (up to 24 months).
• For short-term storage (up to 4 weeks), refrigerate at 2-8C.
• After reconstitution, store at 2-8C and use within 30 days.
• Avoid repeated freeze-thaw cycles.
• Protect from light and moisture at all times.

Certificate of Analysis

Every batch of TB-500 sold by Pepspan undergoes comprehensive third-party testing. Our Certificate of Analysis (COA) includes HPLC purity analysis, mass spectrometry confirmation, endotoxin testing, and sterility verification. COA documentation is available upon request for any batch number. All testing is performed by independent European laboratories to ensure the highest standards of accuracy and reliability.

Current Batch: Pending first shipment

Every batch of TB-500 sold by Pepspan is independently tested by Janoshik Analytical (Prague, Czech Republic). HPLC purity analysis, mass spectrometry, and amino acid sequencing are performed for each shipment. Reports are batch-specific — not generic.

Research Disclaimer

This product is intended for laboratory research use only. It is not a drug, food, cosmetic, or dietary supplement. TB-500 is not approved for human or veterinary use by any regulatory agency. It must not be administered to humans or animals outside of approved clinical trials. By purchasing this product, you confirm that you are a qualified researcher and that this product will be used exclusively for legitimate scientific research purposes. Pepspan assumes no liability for any misuse of this product. All handling must comply with local regulations and institutional safety protocols.